In Vitro and In Vivo Induction, and Characterization of Beauvericin Isolated from Beauveria bassiana and Its Bioassay on Galleria mellonella Larvae
نویسنده
چکیده
Entomopathogenic fungi produce secondary metabolites which may bioactively help fungus in its virulence toward insect hosts. Beauveria bassiana produces several toxic low molecular compounds in vitro as well as in vivo, the most important of them is Beauvericin. The BEH isolate of the fungus was selected for Beauvericin assay. Beauvericin was obtained from surface and submerged cultures of the fungus in PDB and PDA, culture filtrates, and in vivo conidia harvested from insect cadavers. Results indicated that in vivo fungal conidia contained the most Beauvericin, causing a higher mortality to Galleria mellonella larvae as compared with in vitro fungal products in their different concentrations. Beauvericin chromatogram revealed that Beauvericin was in its greatest quantity in comparison with the other secondary metabolites of BEH isolate. The impact of Beauvericin on mean larval survival and on paralysis time was in agreement with bioassay data showing lower ST50 vs. higher PT50 in larvae treated with metabolites of the insect-derived conidia.
منابع مشابه
Pathogenecity of Two Beauveria Bassiana Indigenous Isolates towards the Greater Wax Moth Galleria Mellonella L. Larvae in Egypt
Bioassays were carried out in the laboratory to determine the virulence of two Beauveria bassiana indigenous isolates on the greater wax moth Galleria mellonella. LC50 and LT50 values were taken as indices for the efficacy of fungal isolates, designated as Bb1 and Bb2, collected from Ismailia governorate in Egypt and isolated from soil and trap larvae of G. mellonella, respectively. The larvae ...
متن کاملExpression of the insect metalloproteinase inhibitor IMPI in the fat body of Galleria mellonella exposed to infection with Beauveria bassiana.
The inducible metalloproteinase inhibitor (IMPI) discovered in Galleria mellonella is currently the only specific inhibitor of metalloproteinases found in animals. Its role is to inhibit the activity of metalloproteinases secreted by pathogenic organisms as virulence factors to degrade immune-relevant polypeptides of the infected host. This is a good example of an evolutionary arms race between...
متن کاملThe effects of dietary nickel on the detoxification enzymes, innate immunity and resistance to the fungus Beauveria bassiana in the larvae of the greater wax moth Galleria mellonella.
In this study, we tested the effects of dietary nickel on the activity of glutathione S-transferase (GST), esterases, phenoloxidase, and encapsulation in the haemolymph of larvae of the greater wax moth Galleria mellonella. We also explored the effects of dietary nickel on larval resistance to infection by the fungus Beauveria bassiana. Larvae fed a low dose of nickel (10 μg g(-1)) had signific...
متن کاملPathogenicity of Beauveria Bassiana (deuteromycotina: Hyphomycetes) against the White Grub Laniifera Cyclades (lepidoptera: Pyralidae) under Field and Greenhouse Conditions
The white grub Laniifera cyclades Druce of prickly pear cactus or nopal is a pest that limits the commercial production of Opuntia . The gregarious larvae perforate the cladodes devouring the inner part, thereby forming large galleries until reaching the central axis of the plant; during their movement through the inner part of the cactus, the larvae make orifices to the exterior to expel their...
متن کاملImmunity of the greater wax moth Galleria mellonella.
Investigation of insect immune mechanisms provides important information concerning innate immunity, which in many aspects is conserved in animals. This is one of the reasons why insects serve as model organisms to study virulence mechanisms of human pathogens. From the evolutionary point of view, we also learn a lot about host-pathogen interaction and adaptation of organisms to conditions of l...
متن کامل